Single-cell biophysics<\/span><\/a><\/b> | Membrane biophysics<\/span><\/a><\/b> | Physics of collective behavior<\/span><\/a><\/b> | Biophysical tools<\/span><\/a><\/b><\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div> Recent publications from the CMU biophysics group \u2013 not a complete list. A more comprehensive overview of publications is provided on the group pages of the individual investigators \u2013 and on Google Scholar, the National Library of Medicine (NCBI), ORCID, or Publons (aka ResearcherID). <\/span><\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div> <\/a>Single-Cell Biophysics<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div> Fundamental principles in bacterial physiology\u2014history, recent progress, and the future with focus on cell size control: a review<\/p><\/div>\n\t\t\t<\/div> Suckjoon Jun, Fangwei Si, Rami Pugatch, & Matthew Scott<\/p><\/div>\n\t\t\t<\/div> Reports on Progress in Physics, 81<\/strong>(5), 056601 (2018)<\/a>.<\/p><\/div>\n\t\t\t<\/div> This 64-page article reviews more than 700 references in the history of bacterial physiology since the beginning of the 20th<\/sup> century, providing a comprehensive overview of major ideas and approaches for anyone interested in the fundamental problems in bacterial physiology.<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div> Invariance of initiation mass and predictability of cell size in Escherichia coli<\/em><\/p><\/div>\n\t\t\t<\/div> Fangwei Si, Dongyang Li, Sarah E. Cox, John T. Sauls, Omid Azizi, Cindy Sou, Amy B. Schwartz, Michael J. Erickstad, Yonggyn Jun, Xintian Li, & Suckjoon Jun<\/p><\/div>\n\t\t\t<\/div> Current Biology, 27<\/strong>(9), 1278\u20131287 (2017)<\/a>.<\/p><\/div>\n\t\t\t<\/div> In this paper, we hypothesized that the E. coli<\/em> cell is composed of conceptual \u201cunit cells.\u201d By extensively perturbing the growth physiology in steady-state conditions using genetic and pharmacological methods, we discovered the invariant initiation mass as the basic unit of the cell size. This finding unraveled how cells coordinate their biomass and DNA content by imposing a simple control on replication initiation.<\/p><\/div>\n\t\t\t<\/div> See also the associated commentary in Current Biology<\/a>, as well as the news Coverage by phys.org<\/a>\u00a0and ScienceDaily<\/a>.<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div> Biphasic growth dynamics control cell division in Caulobacter crescentus<\/i><\/p><\/div>\n\t\t\t<\/div> S. Banerjee, K. Lo, M.K.\u00a0Daddysman, A. Selewa, T. Kuntz, A.R. Dinner and N.F. Scherer\u00a0<\/span><\/p><\/div>\n\t\t\t<\/div> Nat. Microbiol. 2<\/strong>, 17116 (2017).<\/a><\/p><\/div>\n\t\t\t<\/div> Cell size is specific to each species and impacts cell function. Here, we investigate size control and the cell cycle dependence of bacterial growth using multigenerational cell growth and shape data for single <\/span>C. crescentus<\/i> cells. Our analysis reveals a biphasic mode of growth: a relative timer phase before cell wall constriction, followed by a pure adder phase during constriction. We present a mathematical model that quantitatively explains this biphasic model for cell size control.<\/span><\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div> Multiscale spatial organization of RNA polymerase in Escherichia coli<\/em><\/p><\/div>\n\t\t\t<\/div> U. Endesfelder, K. Finan, S. Holden, P. Cook, A. Kapanidis, and M. Heilemann<\/p><\/div>\n\t\t\t<\/div> Biophys. J. 105<\/strong>, 172\u2013181 (2013)<\/a><\/p><\/div>\n\t\t\t<\/div> We use photoactivated localization microscopy to localize individual molecules of RNA polymerase (RNAP) in Escherichia coli. In cells growing rapidly in nutrient-rich media, we find that RNAP is organized in 2\u20138 bands. The band number scaled directly with cell size (and so with the chromosome number), and bands often contained clusters of >70 tightly packed RNAPs and clusters of such clusters. In nutrient-poor media, RNAPs were located in only 1\u20132 bands; within these bands, a disproportionate number of RNAPs were found in clusters containing \u223c20\u201350 RNAPs.<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div> <\/a>Membrane Biophysics<\/p><\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div> Spontaneous curvature, differential stress, and bending modulus of asymmetric lipid membranes<\/p><\/div>\n\t\t\t<\/div> A. Hossein and M. Deserno<\/p><\/div>\n\t\t\t<\/div> Biophys. J. 118<\/strong>, 624\u2013642 (2020)<\/a>.<\/p><\/div>\n\t\t\t<\/div> The two leaflets of a lipid bilayer can differ in composition or in stress, but these two degrees of asymmetry are coupled. They can affect curvature and even rigidity of a membrane. This paper discusses the connection, and how it is affected by a lipid species, such as cholesterol, that can flip-flop between leaflets.<\/p><\/div>\n\t\t\t<\/div>![\"\"](\"http:\/\/research.phys.cmu.edu\/biophysics\/wp-content\/uploads\/sites\/2\/2021\/08\/publication-pic-2.png\" "\\"publication")
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